Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy     

CHAN Zhuhua  CHEN Xinglin  HOU Yanping  GAO Boliang  ZHAO Chungui  YANG Suping  ZENG Runying 《海洋学报(英文版)》2018,(2)

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.  相似文献   

黑河中游夏季昼夜水汽同位素特征及水汽来源分析          下载免费PDF全文

孟鸿飞  张明军  王圣杰  邱雪  杜铭霞  张亚宁  余秀秀  周苏娥 《干旱区地理》2020,43(2):360-370

基于2012年6~8月的实测水汽同位素数据及相关气象数据，对黑河中游夏季昼夜的同位素基本特征、水汽来源方向及潜在蒸发源地进行了研究。结果表明：空气水汽线斜率白天大于夜晚和水汽过量氘值白天大于夜晚，综合说明白天局地蒸发较夜晚强烈；夏季受西风水汽影响显著。其中，6月主要受西风水汽和北冰洋水汽影响，7、8月主要受西风水汽和东南方向水汽影响，且8月受东南方向水汽影响最为明显；水汽运移路径上下垫面地形和气压带移动会影响水汽后向轨迹高度，西北方向上水汽输送通道较顺畅，风速较大，有利于水汽的输送；水汽蒸发源地主要集中在研究区周围及以东、以北部，其次是西北部。绿洲是主要的水汽蒸发源地，其次是城市和河流，白天较夜晚局地蒸发强烈且面积大。  相似文献   

敦煌-格尔木铁路沿线风动力环境特征     

鱼燕萍  张克存  安志山  张余 《中国沙漠》2020,40(1):41-48

敦煌-格尔木铁路沿线地形复杂、起沙因素多变、沙源丰富，沙害问题日益严重。目前对其风沙活动规律还未有研究，不利于防沙工作的开展。为此，通过对自北向南的5个观测点（S1、S2、S3、S4、S5）风速和风向的观测、计算和分析，利用平均风速、起沙风况及输沙势对敦格铁路沿线的风动力环境特征进行研究。结果表明：S5、S4和S3的风况对铁路风沙灾害防治意义较大。S5年平均风速、起沙风频率和输沙势最大，春季风沙活动最为强烈，且风向单一、风力强劲，风沙运动方向基本与铁路垂直，沙粒易在铁路附近堆积。S4夏季风沙活动最为强烈；S3春季风沙活动最为强烈，且风向单一，S4和S3的风沙运动方向与铁路夹角小于90°，附近沙源广阔，铁路易受风沙侵蚀，阻碍交通运营。  相似文献   

游客-环境互动视角下旅游者目的地环境恢复性效应     

朱芳  苏勤  陶云  沈惊宏 《热带地理》2020,40(4):636-648

日常生活环境和旅游目的地环境都可以为个体提供恢复的机会，不同环境的属性特征导致不同程度的恢复。为了探究环境类型与恢复性效应之间的关系及停留时间的调节作用，文章采用问卷调查方式，利用配对样本t检验和混合设计方差分析相结合的方法，探讨游客在不同环境中的恢复性效应，并对这一现象提供心理上的解释。结果表明：1）恢复体验的4个维度（放松体验、心理脱离、掌握体验、控制体验）在旅游目的地环境中的得分均高于日常生活环境。2）掌握和控制体验维度在城市旅游目的地中的变化程度高于乡村旅游目的地，两种类型目的地的放松和心理脱离维度差异较小。3）随着在目的地停留时间的延长，乡村旅游目的地放松体验和心理脱离维度变化程度高于城市旅游目的地，两种类型目的地环境恢复性效应会相互靠近。因此，游客的环境恢复性效应不仅受到环境和目的地类型的影响，同时还与游客的适应程度有关，且受到其停留时间的调节作用。  相似文献   

Finding KBO Flyby Targets for New Horizons     

Spencer  John  Buie  Marc  Young  Leslie  Guo  Yanping  Stern  Alan 《Earth, Moon, and Planets》2003,92(1-4):483-491

Development of the New Horizons mission to Pluto and the Kuiper Belt is now fully funded by NASA (Stern and Spencer, this volume). If all goes well, New Horizons will be launched in January 2006, followed by a Jupiter gravity assist in 2007, with Pluto arrival expected in either 2015 or 2016, depending on the launch vehicle chosen. A backup launch date of early 2007, without a Jupiter flyby, would give a Pluto arrival in 2019 or 2020. In either case, a flyby of at least one Kuiper Belt object (KBO) is planned following the Pluto encounter, sometime before the spacecraft reaches a heliocentric distance of 50 AU, in 2021 or 2023 for the 2006 launch, and 2027 or 2029 for the 2007 launch. However, none of the almost 1000 currently-known KBOs will pass close enough to the spacecraft trajectory to be targeted by New Horizons, so the KBO flyby depends on finding a suitable target among the estimated 500,000 KBOs larger than 40 km in diameter. This paper discusses the issues involved in finding one or more KBO targets for New Horizons. The New Horizons team plans its own searches for mission KBOs but will welcome other U.S, or international team who wish to become involved in exchange for mission participation at the KBO.  相似文献   

Three Super Active Regions in the Descending Phase of Solar Cycle 23   总被引：2，自引：0，他引：2  

Hong-Qi Zhang  Xing-Ming Bao  Yin Zhang  Ji-Hong Liu  Shu-Dong Bao  Yuan-Yong Deng  Wei Li  Jie Chen  Jin-Ping Dun  Jiang-Tao Su  Juan Guo  Xiao-Fan Wang  Ke-Liang Hu  Gang-Hua Lin  Dong-Guang WangNational Astronomical Observatories  Chinese Academy of Sciences  Beijing   《中国天文和天体物理学报》2003,3(6):491-494

We analyze the magnetic configurations of three super active regions, NOAA 10484, 10486 and 10488, observed by the Huairou Multi-Channel Solar Telescope (MCST) from 2003 October 18 to November 4. Many energetic phenomena, such as flares (including a X-28 flare) and coronal mass ejections (CMEs), occurred during this period. We think that strong shear and fast emergence of magnetic flux are the main causes of these events. The question is also of great interest why these dramatic eruptions occurred so close together in the descending phase of the solar cycle.  相似文献   

彗星等离子体某些特征的讨论     

李中元  李嘉巍  段素平  陈耀  石志东 《紫金山天文台台刊》2003,(1)

本文讨论了彗星中尘埃粒子的充电机制 ,带电特性和平衡电势的变化规律 ,分析了彗星尘埃的破碎特性和临界半径 ,得出了很有意义的结果  相似文献   

山东温泉旅游资源分析及科学发展思考   总被引：1，自引：0，他引：1  

李宇荣  王艳平 《山东国土资源》2008,24(3):35-38

山东省温泉资源分布广泛、储量大，利用历史十分悠久，在温泉资源开发应用过程中，应根据其分布、类型、开发利用现状，并结合国内外的科技成果而进行科学开发与综合利用，建设既有地方民俗文化特色又与国际接轨的可持续发展模式。  相似文献   

Investigation of Failure Mechanisms and Migration of Organic Chemicals at Wilsonville, Illinois     

B.L. Herzog  R.A. Griffin  C.J. Stohr  L.R. Follmer  W.J. Morse  W.J. Su 《Ground Water Monitoring & Remediation》1989,9(2):82-89

Ground water contamination was discovered in 1981 in a monitoring well at the Earthline disposal facility near Wilsonville, Illinois. Organic chemicals had migrated at a rate 100 to 1000 times greater than predicted when the site received its permit to operate in 1978. Postulated failure mechanisms included migration through previously unmapped permeable zones, subsidence of an underground mine, organic-chemical and clay-mineral interactions, acid-mine drainage and clay interactions, trench-cover settlement, and erosion.
In this investigation, the Illinois State Geological Survey found the primary reason for the rapid migration: the presence of previously undetermined fractures and joints in glacial till. The inaccurate predictions of hydraulic conductivity were based on laboratory-determined values that did not adequately measure the effects of fractures and joints on the transit time calculations. Field-measured hydraulic conductivity values were generally 10 to 1000 times greater than their laboratory-measured counterparts, thus largely accounting for the discrepancy between predicted and actual migration rates in the transit time calculations. The problem was compounded, however, by the burial of liquid wastes and by trench covers that allowed excess surface runoff to enter the trenches. Organic-chemical and clay-mineral interactions may also have exacerbated the problem in areas where liquid organic wastes were buried.  相似文献   

Background concentrations of elements in surface soils and their changes as affected by agriculture use in the desert-oasis ecotone in the middle of Heihe River Basin,North-west China   总被引：1，自引：0，他引：1  

Yong-zhong Su  Rong Yang 《Journal of Geochemical Exploration》2008

The concentrations of twenty four chemical elements in the surface layer of natural desert soils and the cultivated farmland soils were measured at a desert-oasis ecotone in the middle of Heihe river basin, north-west China. Background values were estimated for (a) major elements (Si 335.3 g kg^− 1, Al 49.4 g kg^− 1, Fe 19.1 g kg^− 1, Ca 29.4 g kg^− 1, Mg 8.9 g kg^− 1, K 20.1 g kg^− 1, Na 17.5 g kg^− 1 and P 0.338 g kg^− 1), (b) heavy metals and non-metals (Cr 55.8 mg kg^− 1, Mn 404.8 mg kg^− 1, Ni 17.7 mg kg^− 1, Cu 5.1 mg kg^− 1, Zn 33.7 mg kg^− 1, Pb 15.5 mg kg^− 1 and As 5.2 mg kg^− 1) and (c) other trace elements (Ti 2.0 mg kg^− 1, V 55.3 mg kg^− 1, Co 5.7 mg kg^− 1, Rb 82.4 mg kg^− 1, Sr 232.9 mg kg^− 1, Y 14.7 mg kg^− 1, Zr 194.9 mg kg^− 1, Nb 7.8 mg kg^− 1 and Ba 720.6 mg kg^− 1). After natural desert soil was cultivated for agricultural use, significant changes in element concentrations occurred under tillage, irrigation and fertilisation management. Compared to natural soil, the for the levels of Si, K, Na, Sr, Zr and Ba decreased, and no changes were observed for Rb, while the values of the other 17 elements increase in agricultural soil from 1.2 to 3.5 times. However, their absolute concentrations are still low, suggesting that the arable soil in this region remains comparatively a clean soil. The increased silt, clay and organic carbon content, under long-term irrigation, enriched the fine-grained materials, and application of fertilisers and manure contributed to the accumulation of most elements in arable soil. The accumulation of elements in agricultural soil increased with increasing cultivation years and extent of soil development.  相似文献